Ontology highlight
ABSTRACT:
SUBMITTER: Chmura AJ
PROVIDER: S-EPMC37461 | biostudies-literature | 2001 Jul
REPOSITORIES: biostudies-literature
Chmura A J AJ Orton M S MS Meares C F CF
Proceedings of the National Academy of Sciences of the United States of America 20010710 15
Here we report an approach to the design and production of antibody/ligand pairs, to achieve functional affinity far greater than avidin/biotin. Using fundamental chemical principles, we have developed antibody/ligand pairs that retain the binding specificity of the antibody, but do not dissociate. Choosing a structurally characterized antibody/ligand pair as an example, we engineered complementary reactive groups in the antibody binding pocket and the ligand, so that they would be in close prox ...[more]