Engineered nanostructured ?-sheet peptides protect membrane proteins.
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ABSTRACT:
SUBMITTER: Tao H
PROVIDER: S-EPMC3753066 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Publications
Engineered nanostructured β-sheet peptides protect membrane proteins.
Tao Houchao H Lee Sung Chang SC Moeller Arne A Roy Rituparna Sinha RS Siu Fai Yiu FY Zimmermann Jörg J Stevens Raymond C RC Potter Clinton S CS Carragher Bridget B Zhang Qinghai Q
Nature methods 20130630 8
We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding casse ...[more]
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