Ontology highlight
ABSTRACT:
SUBMITTER: Zhang X
PROVIDER: S-EPMC3753654 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Zhang Xuemei X Song Yang Y Shah Akash Y AY Lekova Virzhiniya V Raj Arjun A Huang Ling L Behlke Mark A MA Tsourkas Andrew A
Nucleic acids research 20130627 15
Recently, we developed an oligonucleotide-based probe, ratiometric bimolecular beacon (RBMB), which generates a detectable fluorescent signal in living cells that express the target RNA. Here, we show that RBMBs can also be used to image single RNA transcripts in living cells, when the target RNA is engineered to contain as few as four hybridization sites. Moreover, comparison with single-molecule fluorescence in situ hybridization confirmed that RBMBs could be used to accurately quantify the nu ...[more]