Strain/species-specific probe design for microbial identification microarrays.
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ABSTRACT:
SUBMITTER: Tu Q
PROVIDER: S-EPMC3754697 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Publications
Strain/species-specific probe design for microbial identification microarrays.
Tu Qichao Q He Zhili Z Deng Ye Y Zhou Jizhong J
Applied and environmental microbiology 20130607 16
Specific identification of microorganisms in the environment is important but challenging, especially at the species/strain level. Here, we have developed a novel k-mer-based approach to select strain/species-specific probes for microbial identification with diagnostic microarrays. Application of this approach to human microbiome genomes showed that multiple (≥10 probes per strain) strain-specific 50-mer oligonucleotide probes could be designed for 2,012 of 3,421 bacterial strains of the human m ...[more]
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