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Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-?-hydroxybutyrate.

ABSTRACT: A novel facultative psychrotroph (strain CBS-1), which accumulates poly-?-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

SUBMITTER: Li R

PROVIDER: S-EPMC3755782 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

Li Rongpeng R Jiang Yuji Y Wang Xinfeng X Yang Jingjing J Gao Yuan Y Zi Xiaoli X Zhang Xia X Gao Haofeng H Hu Nan N

SpringerPlus 20130723

A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew op ...[more]

PMID: 24010027

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