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A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.

ABSTRACT: We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Åvia on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.

SUBMITTER: Perry SL

PROVIDER: S-EPMC3755953 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.

Perry Sarah L SL Guha Sudipto S Pawate Ashtamurthy S AS Bhaskarla Amrit A Agarwal Vinayak V Nair Satish K SK Kenis Paul J A PJ

Lab on a chip 20130705 16

We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Åvia on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods. ...[more]

PMID: 23828485

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