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Transcriptional dynamics of endodermal organ formation.


ABSTRACT: Although endodermal organs including the liver, pancreas, and intestine are of significant therapeutic interest, the mechanism by which the endoderm is divided into organ domains during embryogenesis is not well understood. To better understand this process, global gene expression profiling was performed on early endodermal organ domains. This global analysis was followed up by dynamic immunofluorescence analysis of key transcription factors, uncovering novel expression patterns as well as cell surface proteins that allow prospective isolation of specific endodermal organ domains. Additionally, a repressive interaction between Cdx2 and Sox2 was found to occur at the prospective stomach-intestine border, with the hepatic and pancreatic domains forming at this boundary, and Hlxb9 was revealed to have graded expression along the dorsal-ventral axis. These results contribute to understanding the mechanism of endodermal organogenesis and should assist efforts to replicate this process using pluripotent stem cells.

SUBMITTER: Sherwood RI 

PROVIDER: S-EPMC3756511 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Transcriptional dynamics of endodermal organ formation.

Sherwood Richard I RI   Chen Tzong-Yang Albert TY   Melton Douglas A DA  

Developmental dynamics : an official publication of the American Association of Anatomists 20090101 1


Although endodermal organs including the liver, pancreas, and intestine are of significant therapeutic interest, the mechanism by which the endoderm is divided into organ domains during embryogenesis is not well understood. To better understand this process, global gene expression profiling was performed on early endodermal organ domains. This global analysis was followed up by dynamic immunofluorescence analysis of key transcription factors, uncovering novel expression patterns as well as cell  ...[more]

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