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Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.

ABSTRACT: OBJECTIVES:To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root. MATERIALS AND METHODS:The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data. RESULTS:The active fraction of S. tetragonum showed presence of anti-diabetes mellitus activity in type-2 diabetic rats. It did not significantly influence insulin release from cultured islets. Two active principles (active at 2 mg/kg dose) were isolated and characterized with spectral data. One of them was identified as an iridoid type glycoside and the other one was a lapachol like compound (derivative of naphthoquinone). CONCLUSIONS:Two active principles from the anti-diabetes fraction of S. tetragonum root were isolated and identified as an iridoid glycoside and a naphthoquinone derivative.

SUBMITTER: Bino Kingsley R

PROVIDER: S-EPMC3758101 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.

Bino Kingsley Renjit R Mishra Manisha M Brindha Pemaiah P Subramoniam Appian A

Journal of young pharmacists : JYP 20130320 1

<h4>Objectives</h4>To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root.<h4>Materials and methods</h4>The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data.<h4>Results</h4>The active fraction of S. tetragonu ...[more]

PMID: 24023445

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Project description:Background:The flower of Hagenia abyssinica (Rosaceae) has been used in traditional health systems to treat diabetes mellitus in Ethiopia and Tanzania. However, the antidiabetic activity of this medicinal plant is not scientifically validated and authenticated. The present study aimed to investigate the in vitro and in vivo anti-diabetic activity of flower crude extract and solvent fractions of Hagenia abyssinica. Methods:The in vitro ?-amylase inhibition and antioxidant activity of the crude extract and solvent fractions of Hagenia abyssinica were evaluated by using 3,5-dinitrosalicylic acid (DNSA) and diphenyl-2-picrylhydrazyl (DPPH) assay model, respectively. Blood glucose lowering activity of 80% methanolic flower crude extract and solvent fraction was studied in four animal models: normoglycemic mice model, oral glucose loaded mice model, single dose-treated streptozotocin-induced diabetic mice model, and repeated dose-treated streptozotocin-induced diabetic mice model. The effect of the crude extract and solvent fraction of Hagenia abyssinica on diabetic lipid profile and body weight was also studied. Results:The acute toxicity study of Hagenia abyssinica flower extract did not show mortality in the animals at the limit dose of 2g/kg during the observation period. The result of ?-amylase enzyme inhibition activity was found in a dose-dependent manner, the strongest activity was shown by ethyl acetate fraction (54.23% inhibition at 800 ?g/mL) compared to the standard acarbose having 91.87% inhibition at 800 ?g/mL. Among these extracts, the crude extract had the highest antioxidant activity (58.38% inhibition at 500 ?g/mL). The crude extract of H. abyssinica showed significant blood glucose-lowering effect on normoglycemic mice and oral glucose loaded mice. In streptozotocin-induced diabetic mice model, the crude extract and ethyl acetate fraction significantly decreased the fasting blood glucose level after 14 days of treatment. There were significant reductions in serum total cholesterol, serum triglycerides, very low-density lipoprotein, and low-density lipoprotein. However, there were significant increments in body weight and high-density lipoprotein as compared to untreated diabetic mice. Conclusion:The result demonstrated the beneficial biochemical effects of Hagenia abyssinica extract by inhibiting ?-amylase, scavenging diphenyl-2-picrylhydrazyl (DPPH) and improving serum lipid profile levels. The flower crude extract and solvent fractions of Hagenia abyssinica are effective in lowering blood glucose levels in diabetic and normoglycemic mice. The claimed traditional use as antidiabetic has scientific ground.

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Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.

Publications

Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.

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