Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.
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ABSTRACT:
SUBMITTER: Yu Z
PROVIDER: S-EPMC3761309 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.
Yu Zhongsheng Z Ren Mengda M Wang Zhanxiang Z Zhang Bo B Rong Yikang S YS Jiao Renjie R Gao Guanjun G
Genetics 20130705 1
We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila. ...[more]
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