Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
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ABSTRACT:
SUBMITTER: Wagner JC
PROVIDER: S-EPMC4199390 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Publications
Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.
Wagner Jeffrey C JC Platt Randall J RJ Goldfless Stephen J SJ Zhang Feng F Niles Jacquin C JC
Nature methods 20140810 9
Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption f ...[more]
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