Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe S
PROVIDER: S-EPMC3762212 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Watanabe Shigeki S Liu Qiang Q Davis M Wayne MW Hollopeter Gunther G Thomas Nikita N Jorgensen Nels B NB Jorgensen Erik M EM
eLife 20130903
Synaptic vesicles can be released at extremely high rates, which places an extraordinary demand on the recycling machinery. Previous ultrastructural studies of vesicle recycling were conducted in dissected preparations using an intense stimulation to maximize the probability of release. Here, a single light stimulus was applied to motor neurons in intact Caenorhabditis elegans nematodes expressing channelrhodopsin, and the animals rapidly frozen. We found that docked vesicles fuse along a broad ...[more]