Ontology highlight
ABSTRACT:
SUBMITTER: Nour AM
PROVIDER: S-EPMC3764215 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Nour Adel M AM Li Yue Y Wolenski Joseph J Modis Yorgo Y
PLoS pathogens 20130905 9
Flaviviruses deliver their genome into the cell by fusing the viral lipid membrane to an endosomal membrane. The sequence and kinetics of the steps required for nucleocapsid delivery into the cytoplasm remain unclear. Here we dissect the cell entry pathway of virions and virus-like particles from two flaviviruses using single-particle tracking in live cells, a biochemical membrane fusion assay and virus infectivity assays. We show that the virus particles fuse with a small endosomal compartment ...[more]