Validation and application of a new reversed phase HPLC method for in vitro dissolution studies of rabeprazole sodium in delayed-release tablets.
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ABSTRACT: The purpose of this study was to develop and validate a new reversed phase high performance liquid chromatographic (RP-HPLC) method to quantify in vitro dissolution assay of rabeprazole sodium in pharmaceutical tablet dosage form. Method development was performed on C 18, 100 × 4.6?mm ID, and 10? ? m particle size column, and injection volume was 20? ? L using a diode array detector (DAD) to monitor the detection at 280?nm. The mobile phase consisted of buffer: acetonitrile at a ratio of 60?:?40 (v/v), and the flow rate was maintained at 1.0?mL/min. The method was validated in terms of suitability, linearity, specificity, accuracy, precision, stability, and sensitivity. Linearity was observed over the range of concentration 0.05-12.0? ? g/mL, and the correlation coefficient was found excellent >0.999. The method was specific with respect to rabeprazole sodium, and the peak purity was found 99.99%. The method was precise and had relative standard deviations (RSD) less than 2%. Accuracy was found in the range of 99.9 to 101.9%. The method was robust in different variable conditions and reproducible. This proposed fast, reliable, cost-effective method can be used as quality control tool for the estimation of rabeprazole sodium in routine dissolution test analysis.
SUBMITTER: Nawaz MS
PROVIDER: S-EPMC3766580 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
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