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Differentiation of bovine coronavirus (bcov) genotypes by a restriction enzyme assay.

ABSTRACT: This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.

SUBMITTER: Pinheiro de Souza S 

PROVIDER: S-EPMC3768659 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Differentiation of bovine coronavirus (bcov) genotypes by a restriction enzyme assay.

Pinheiro de Souza Sibele S   Miyuki Asano Karen K   Lucas Sandri Thaisa T   Nunes de Barros Iracema I   José Richtzenhain Leonardo L   Eduardo Brandão Paulo P  

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20100701 3

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation. ...[more]

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