Ontology highlight
ABSTRACT:
SUBMITTER: Roy R
PROVIDER: S-EPMC3769523 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Roy Rahul R Hohng Sungchul S Ha Taekjip T
Nature methods 20080601 6
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by biophysicists. We provide a practical guide to using smFRET, focusing on the study of immobilized molecules that allow measurements of single-molecule reaction trajectories from 1 ms to many minutes. We discuss issu ...[more]