Ontology highlight
ABSTRACT:
SUBMITTER: Bazewicz CG
PROVIDER: S-EPMC3769706 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
The journal of physical chemistry. B 20130718 30
We have synthesized the unnatural amino acid (UAA), 4-azidomethyl-L-phenylalanine (pN₃CH₂Phe), to serve as an effective vibrational reporter of local protein environments. The position, extinction coefficient, and sensitivity to local environment of the azide asymmetric stretch vibration of pN₃CH₂Phe are compared to the vibrational reporters: 4-cyano-L-phenylalanine (pCNPhe) and 4-azido-L-phenylalanine (pN₃Phe). This UAA was genetically incorporated in a site-specific manner utilizing an enginee ...[more]