Ontology highlight
ABSTRACT:
SUBMITTER: Fu Y
PROVIDER: S-EPMC3773023 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Fu Yanfang Y Foden Jennifer A JA Khayter Cyd C Maeder Morgan L ML Reyon Deepak D Joung J Keith JK Sander Jeffry D JD
Nature biotechnology 20130623 9
Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of CRISPR-associated (Cas)9-based RGNs. We find that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface. We also readily detected off-target alterations induced by f ...[more]