Ontology highlight
ABSTRACT:
SUBMITTER: Yea K
PROVIDER: S-EPMC3773771 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Yea Kyungmoo K Zhang Hongkai H Xie Jia J Jones Teresa M TM Yang Guang G Song Byeong Doo BD Lerner Richard A RA
Proceedings of the National Academy of Sciences of the United States of America 20130826 37
When combinatorial antibody libraries are rendered infectious for eukaryotic cells, the integrated antibody genotype and cellular phenotype become permanently linked and each cell becomes a selection system unto itself. These systems should be ideal for the identification of proteins and pathways that regulate differentiation so long as selection systems can be devised. Here we use a selection system based on the ability of secreted antibodies to alter the morphology of colonies expressing them ...[more]