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In situ recognition of cell-surface glycans and targeted imaging of cancer cells.

ABSTRACT: Fluorescent sensors capable of recognizing cancer-associated glycans, such as sialyl Lewis X (sLe(x)) tetrasaccharide, have great potential for cancer diagnosis and therapy. Studies on water-soluble and biocompatible sensors for in situ recognition of cancer-associated glycans in live cells and targeted imaging of cancer cells are very limited at present. Here we report boronic acid-functionalized peptide-based fluorescent sensors (BPFSs) for in situ recognition and differentiation of cancer-associated glycans, as well as targeted imaging of cancer cells. By screening BPFSs with different structures, it was demonstrated that BPFS₁ with a FRGDF peptide could recognize cell-surface glycan of sLe(x) with high specificity and thereafter fluorescently label and discriminate cancer cells through the cooperation with the specific recognition between RGD and integrins. The newly developed peptide-based sensor will find great potential as a fluorescent probe for cancer diagnosis.

SUBMITTER: Xu XD

PROVIDER: S-EPMC3775094 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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In situ recognition of cell-surface glycans and targeted imaging of cancer cells.

Xu Xiao-Ding XD Cheng Han H Chen Wei-Hai WH Cheng Si-Xue SX Zhuo Ren-Xi RX Zhang Xian-Zheng XZ

Scientific reports 20130101

Fluorescent sensors capable of recognizing cancer-associated glycans, such as sialyl Lewis X (sLe(x)) tetrasaccharide, have great potential for cancer diagnosis and therapy. Studies on water-soluble and biocompatible sensors for in situ recognition of cancer-associated glycans in live cells and targeted imaging of cancer cells are very limited at present. Here we report boronic acid-functionalized peptide-based fluorescent sensors (BPFSs) for in situ recognition and differentiation of cancer-ass ...[more]

PMID: 24042097

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