Unknown

Dataset Information

0

Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo.


ABSTRACT: Posttranslational modification with small ubiquitin-like modifier (Sumo) regulates numerous cellular and developmental processes. Sumoylation is dynamic with deconjugation by Sumo-specific proteases (Senps) regulating steady-state levels. Different Senps are found in distinct subcellular domains, which may limit their deconjugation activity to colocalizing Sumo-modified proteins. In vitro, Senps can discriminate between the different Sumo paralogs: Sumo1 versus the highly related Sumo2 and Sumo3 (Sumo2/3), which can form poly-Sumo chains. However, a full understanding of Senp specificity in vivo is still lacking. Here, using biochemical and genetic approaches, we establish that Senp1 has an essential, nonredundant function to desumoylate Sumo1-modified proteins during mouse embryonic development. Senp1 specificity for Sumo1 conjugates represents an intrinsic function and not simply a product of colocalization. In contrast, Senp1 has only a limited role in Sumo2/3 desumoylation, although it may regulate Sumo1-mediated termination of poly-Sumo2/3 chains.

SUBMITTER: Sharma P 

PROVIDER: S-EPMC3775507 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo.

Sharma Prashant P   Yamada Satoru S   Lualdi Margaret M   Dasso Mary M   Kuehn Michael R MR  

Cell reports 20130516 5


Posttranslational modification with small ubiquitin-like modifier (Sumo) regulates numerous cellular and developmental processes. Sumoylation is dynamic with deconjugation by Sumo-specific proteases (Senps) regulating steady-state levels. Different Senps are found in distinct subcellular domains, which may limit their deconjugation activity to colocalizing Sumo-modified proteins. In vitro, Senps can discriminate between the different Sumo paralogs: Sumo1 versus the highly related Sumo2 and Sumo3  ...[more]

Similar Datasets

| S-EPMC4197045 | biostudies-literature
| S-EPMC6042150 | biostudies-literature
| S-EPMC6497105 | biostudies-literature
| S-EPMC5775435 | biostudies-literature
| S-EPMC2613119 | biostudies-literature
| S-EPMC8277905 | biostudies-literature
2021-12-07 | GSE163884 | GEO
| S-EPMC5030089 | biostudies-literature
2013-12-31 | GSE47951 | GEO
| S-EPMC8179221 | biostudies-literature