Construction of a thiamin sensor from the periplasmic thiamin binding protein.
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ABSTRACT:
SUBMITTER: Hanes JW
PROVIDER: S-EPMC3776480 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Publications
Construction of a thiamin sensor from the periplasmic thiamin binding protein.
Hanes Jeremiah W JW Chatterjee Debashree D Soriano Erika V EV Ealick Steven E SE Begley Tadhg P TP
Chemical communications (Cambridge, England) 20101202 8
This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity. ...[more]
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