Ontology highlight
ABSTRACT:
SUBMITTER: Manosas M
PROVIDER: S-EPMC3778716 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Manosas Maria M Perumal Senthil K SK Bianco Piero R PR Ritort Felix F Benkovic Stephen J SJ Croquette Vincent V
Nature communications 20130101
Helicases that both unwind and rewind DNA have central roles in DNA repair and genetic recombination. In contrast to unwinding, DNA rewinding by helicases has proved difficult to characterize biochemically because of its thermodynamically downhill nature. Here we use single-molecule assays to mechanically destabilize a DNA molecule and follow, in real time, unwinding and rewinding by two DNA repair helicases, bacteriophage T4 UvsW and Escherichia coli RecG. We find that both enzymes are robust r ...[more]