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Association analysis of two candidate polymorphisms in the tumour necrosis factor-? gene with osteoarthritis in a Chinese population.

ABSTRACT: PURPOSE: The purpose of this study was to investigate the possible association between single nucleotide polymorphisms (SNPs) rs1800629 (TNF-? -308) and rs361525 (TNF-? -238) of the tumour necrosis factor (TNF)-? gene and susceptibility to osteoarthritis (OA) in the Han Chinese population. METHODS: The TNF-? -308 and -238 genotypes were determined by TaqMan assay in 200 OA cases and 305 controls. Odds ratios (ORs) for OA and 95% confidence intervals (CIs) from unconditional logistic regression models were used to evaluate relative risks. RESULTS: The frequencies of the allele 'A' of rs1800629 were 16% and 8.85% in OA cases and in controls, respectively, and thus the -308A allele had a 1.9612-fold (95% CI = 1.3323-2.8869, P < 0.001) increased risk for OA as compared to the -308G allele. However, no significant differences were found in the genotype and allele frequencies for rs361525 between OA and HC groups. CONCLUSIONS: In the Han Chinese population, the allele 'A' of TNF-? -308 may increase the risk for OA, whereas TNF-? -238 polymorphisms do not play a role in OA patients.

SUBMITTER: Ji B

PROVIDER: S-EPMC3779584 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Association analysis of two candidate polymorphisms in the tumour necrosis factor-α gene with osteoarthritis in a Chinese population.

Ji Bin B Shi Jixiang J Cheng Xiangyu X Zhou Junjie J Zhou Qiang Q Cao Chengfu C Pang Jinhui J

International orthopaedics 20130609 10

<h4>Purpose</h4>The purpose of this study was to investigate the possible association between single nucleotide polymorphisms (SNPs) rs1800629 (TNF-α -308) and rs361525 (TNF-α -238) of the tumour necrosis factor (TNF)-α gene and susceptibility to osteoarthritis (OA) in the Han Chinese population.<h4>Methods</h4>The TNF-α -308 and -238 genotypes were determined by TaqMan assay in 200 OA cases and 305 controls. Odds ratios (ORs) for OA and 95% confidence intervals (CIs) from unconditional logistic ...[more]

PMID: 23748461

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Project description:BackgroundThe humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha.AimsTo investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels.Study designCase-control study.MethodsSeventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction-restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay.ResultsWe observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05).ConclusionOur results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsis.

Dataset Information

Association analysis of two candidate polymorphisms in the tumour necrosis factor-? gene with osteoarthritis in a Chinese population.

Publications

Association analysis of two candidate polymorphisms in the tumour necrosis factor-α gene with osteoarthritis in a Chinese population.

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