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ABSTRACT: Objectives
This case-controlled study aimed to identify the association of tumor necrosis factor (TNF)α-1031 and TNFβ+ 252 gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with factors related to CRS.Methods
All deoxyribonucleic acid (DNA) samples were genotyped for TNFα-1031 and TNFβ+252 genes by mean of polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP). The statistical analysis were carried out using chi-square test or Fisher exact test to determine the associations of these gene polymorphisms in CRS. Multiple logistic regression was performed to evaluate the associations of these gene polymorphisms in CRS and its related risk factors.Results
The genotype and allele frequencies of TNFα-1031 and TNFβ+252 gene did not show any significant associations between CRS and healthy controls. However, a significantly statistical difference of TNFα-1031 was observed in CRS participants with atopy (P-value, 0.045; odds ratio, 3.66) but not in CRS with asthma or aspirin intolerance.Conclusion
Although the presence of TNFα-1031 and TNFβ+252 gene polymorphisms did not render any significant associations between CRS and healthy control, this study suggests that TNFα-1031 gene polymorphisms in CRS patients with atopy may be associated with increase susceptibility towards CRS.
SUBMITTER: Misron K
PROVIDER: S-EPMC5545697 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Misron Khairunnisak K Hamid Suzina Sheikh Ab SSA Ahmad Azlina A Ramli Ramiza Ramza RR
Clinical and experimental otorhinolaryngology 20170428 3
<h4>Objectives</h4>This case-controlled study aimed to identify the association of tumor necrosis factor (<i>TNF</i>)<i>α</i>-<i>1031</i> and <i>TNFβ</i>+ <i>252</i> gene polymorphisms between chronic rhinosinusitis (CRS) and healthy controls. Another purpose of this study was to investigate the associations of these gene polymorphisms with factors related to CRS.<h4>Methods</h4>All deoxyribonucleic acid (DNA) samples were genotyped for <i>TNFα-1031</i> and <i>TNFβ+252</i> genes by mean of polym ...[more]