Ontology highlight
ABSTRACT:
SUBMITTER: Jin Y
PROVIDER: S-EPMC3783183 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Jin Ye Y Wu Jianting J Li Yannan Y Cai Zhiming Z Huang Jian-Dong JD
Nucleic acids research 20130709 17
Translation of the sigma factor RpoS is activated by DsrA, RprA and ArcA, three small non-coding sRNAs (sRNA) that expose the ribosome-binding site (RBS) by opening up an inhibitory loop. In the RpoS network, no sRNAs have been found to pair with the RBS, a most common sRNA target site in bacteria. Here, we generate Ribo-0, an artificial sRNA, which represses rpoS translation by pairing with the RBS. Ribo-0 bypasses the RNA chaperon Hfq but requires the RBS to be loosely blocked. Ribo-0 interact ...[more]