A flexible approach to studying post-transcriptional gene regulation in stably transfected mammalian cells.
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ABSTRACT:
SUBMITTER: Nichols RC
PROVIDER: S-EPMC3786348 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
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A flexible approach to studying post-transcriptional gene regulation in stably transfected mammalian cells.
Nichols Ralph C RC Botson John J Wang Xiao Wei XW Hamilton B JoNell BJ Collins Jane E JE Uribe Victoria V Brooks Seth A SA Zan Moe M Rigby William F C WF
Molecular biotechnology 20110701 3
The study of post-transcriptional regulation is constrained by the technical limitations associated with both transient and stable transfection of chimeric reporter plasmids examining the activity of 3'-UTR cis-acting elements. We report the adaptation of a commercially available system that enables consistent stable integration of chimeric reporter cDNA into a single genomic site in which transcription is induced by tetracycline. Using this system, we demonstrate the tight control afforded by t ...[more]
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