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Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.

ABSTRACT: X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

SUBMITTER: Lomb L 

PROVIDER: S-EPMC3786679 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.

Lomb Lukas L   Barends Thomas R M TR   Kassemeyer Stephan S   Aquila Andrew A   Epp Sascha W SW   Erk Benjamin B   Foucar Lutz L   Hartmann Robert R   Rudek Benedikt B   Rolles Daniel D   Rudenko Artem A   Shoeman Robert L RL   Andreasson Jakob J   Bajt Sasa S   Barthelmess Miriam M   Barty Anton A   Bogan Michael J MJ   Bostedt Christoph C   Bozek John D JD   Caleman Carl C   Coffee Ryan R   Coppola Nicola N   Deponte Daniel P DP   Doak R Bruce RB   Ekeberg Tomas T   Fleckenstein Holger H   Fromme Petra P   Gebhardt Maike M   Graafsma Heinz H   Gumprecht Lars L   Hampton Christina Y CY   Hartmann Andreas A   Hauser Günter G   Hirsemann Helmut H   Holl Peter P   Holton James M JM   Hunter Mark S MS   Kabsch Wolfgang W   Kimmel Nils N   Kirian Richard A RA   Liang Mengning M   Maia Filipe R N C FR   Meinhart Anton A   Marchesini Stefano S   Martin Andrew V AV   Nass Karol K   Reich Christian C   Schulz Joachim J   Seibert M Marvin MM   Sierra Raymond R   Soltau Heike H   Spence John C H JC   Steinbrener Jan J   Stellato Francesco F   Stern Stephan S   Timneanu Nicusor N   Wang Xiaoyu X   Weidenspointner Georg G   Weierstall Uwe U   White Thomas A TA   Wunderer Cornelia C   Chapman Henry N HN   Ullrich Joachim J   Strüder Lothar L   Schlichting Ilme I  

Physical review. B, Condensed matter and materials physics 20111201 21

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses  ...[more]

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