Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

ABSTRACT: The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

SUBMITTER: Sugahara M 

PROVIDER: S-EPMC4834484 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature