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Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

ABSTRACT: The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

SUBMITTER: Sugahara M

PROVIDER: S-EPMC4834484 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

Sugahara Michihiro M Song Changyong C Suzuki Mamoru M Masuda Tetsuya T Inoue Shigeyuki S Nakane Takanori T Yumoto Fumiaki F Nango Eriko E Tanaka Rie R Tono Kensuke K Joti Yasumasa Y Kameshima Takashi T Hatsui Takaki T Yabashi Makina M Nureki Osamu O Numata Keiji K Iwata So S

Scientific reports 20160418

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution. ...[more]

PMID: 27087008

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