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Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.

ABSTRACT: Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and Absolute protein expression (APEX) analyses. Forty-one proteins were further identified by two-dimensional gel electrophoresis. Some proteins were revealed to be significantly associated with various metabolic processes, organic solute transport, detoxification and innate immunity. Our results might lay a good foundation for future functional studies of MTs in silkworm and other lepidoptera.

SUBMITTER: Zhong XW

PROVIDER: S-EPMC3787086 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Proteomic-based insight into Malpighian tubules of silkworm Bombyx mori.

Zhong Xiao-wu XW Zou Yong Y Liu Shi-ping SP Yi Qi-ying QY Hu Cui-mei CM Wang Chen C Xia Qing-you QY Zhao Ping P

PloS one 20130930 9

Malpighian tubules (MTs) are highly specific organs of arthropods (Insecta, Myriapoda and Arachnida) for excretion and osmoregulation. In order to highlight the important genes and pathways involved in multi-functions of MTs, we performed a systematic proteomic analysis of silkworm MTs in the present work. Totally, 1,367 proteins were identified by one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry, and as well as by Trans Proteomic Pipeline (TPP) and ...[more]

PMID: 24098719

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