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Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori).


ABSTRACT: The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were identified in this study; and among them, 69 proteins were observed in midgut for the first time. We also found that the silkworm larval midgut responded to starvation by producing a 10?kDa heat shock protein and a diapause hormone precursor.

SUBMITTER: Zhang S 

PROVIDER: S-EPMC3115381 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature

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Proteomic Analysis of Larval Midgut from the Silkworm (Bombyx mori).

Zhang Sai S   Xu Yunmin Y   Fu Qiang Q   Jia Ling L   Xiang Zhonghuai Z   He Ningjia N  

Comparative and functional genomics 20110518


The midgut is the major organ for food digestion, nutrient absorption and also a barrier for foreign substance. The 5th-instar larval stage of silkworm is very important for larval growth, development, and silk production. In the present study, we used 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to analyze the midgut proteins from the 5th-instar larvae as well as the midgut proteins under starvation condition. A total of 96 proteins were i  ...[more]

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