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High-resolution protein structure determination by serial femtosecond crystallography.

ABSTRACT: Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

SUBMITTER: Boutet S 

PROVIDER: S-EPMC3788707 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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High-resolution protein structure determination by serial femtosecond crystallography.

Boutet Sébastien S   Lomb Lukas L   Williams Garth J GJ   Barends Thomas R M TR   Aquila Andrew A   Doak R Bruce RB   Weierstall Uwe U   DePonte Daniel P DP   Steinbrener Jan J   Shoeman Robert L RL   Messerschmidt Marc M   Barty Anton A   White Thomas A TA   Kassemeyer Stephan S   Kirian Richard A RA   Seibert M Marvin MM   Montanez Paul A PA   Kenney Chris C   Herbst Ryan R   Hart Philip P   Pines Jack J   Haller Gunther G   Gruner Sol M SM   Philipp Hugh T HT   Tate Mark W MW   Hromalik Marianne M   Koerner Lucas J LJ   van Bakel Niels N   Morse John J   Ghonsalves Wilfred W   Arnlund David D   Bogan Michael J MJ   Caleman Carl C   Fromme Raimund R   Hampton Christina Y CY   Hunter Mark S MS   Johansson Linda C LC   Katona Gergely G   Kupitz Christopher C   Liang Mengning M   Martin Andrew V AV   Nass Karol K   Redecke Lars L   Stellato Francesco F   Timneanu Nicusor N   Wang Dingjie D   Zatsepin Nadia A NA   Schafer Donald D   Defever James J   Neutze Richard R   Fromme Petra P   Spence John C H JC   Chapman Henry N HN   Schlichting Ilme I  

Science (New York, N.Y.) 20120531 6092

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with s  ...[more]

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