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Serial Femtosecond Crystallography of G Protein-Coupled Receptors.

ABSTRACT: G protein-coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural studies of GPCRs and other challenging proteins by overcoming radiation damage and providing access to high-resolution structures and dynamics using micrometer-sized crystals. Here, we summarize key technology advancements and major milestones of GPCR research using XFELs and provide a brief outlook on future developments in the field.

SUBMITTER: Stauch B

PROVIDER: S-EPMC6290114 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Serial Femtosecond Crystallography of G Protein-Coupled Receptors.

Stauch Benjamin B Cherezov Vadim V

Annual review of biophysics 20180315

G protein-coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural st ...[more]

PMID: 29543504

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