Dataset Information

In vivo T cell activation induces the formation of CD209(+) PDL-2(+) dendritic cells.

ABSTRACT: Two critical functions of dendritic cells (DC) are to activate and functionally polarize T cells. Activated T cells can, in turn, influence DC maturation, although their effect on de novo DC development is poorly understood. Here we report that activation of T cells in mice, with either an anti-CD3 antibody or super antigen, drives the rapid formation of CD209(+)CD11b(+)CD11c(+) MHC II(+) DC from monocytic precursors (Mo-DC). GM-CSF is produced by T cells following activation, but surprisingly, it is not required for the formation of CD209(+) Mo-DC. CD40L, however, is critical for the full induction of Mo-DC following T cell activation. T cell induced CD209(+) Mo-DC are comparable to conventional CD209(-) DC in their ability to stimulate T cell proliferation. However, in contrast to conventional CD209(-) DC, CD209(+) Mo-DC fail to effectively polarize T cells, as indicated by a paucity of T cell cytokine production. The inability of CD209(+) Mo-DC to polarize T cells is partly explained by increased expression of PDL-2, since blockade of this molecule restores some polarizing capacity to the Mo-DC. These findings expand the range of signals capable of driving Mo-DC differentiation in vivo beyond exogenous microbial factors to include endogenous factors produced following T cell activation.

SUBMITTER: Davidson MG

PROVIDER: S-EPMC3788745 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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In vivo T cell activation induces the formation of CD209(+) PDL-2(+) dendritic cells.

Davidson Matthew G MG Alonso Michael N MN Kenkel Justin A JA Suhoski Megan M MM González Joseph C JC Yuan Robert R Engleman Edgar G EG

PloS one 20131002 10

Two critical functions of dendritic cells (DC) are to activate and functionally polarize T cells. Activated T cells can, in turn, influence DC maturation, although their effect on de novo DC development is poorly understood. Here we report that activation of T cells in mice, with either an anti-CD3 antibody or super antigen, drives the rapid formation of CD209(+)CD11b(+)CD11c(+) MHC II(+) DC from monocytic precursors (Mo-DC). GM-CSF is produced by T cells following activation, but surprisingly, ...[more]

PMID: 24098455

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Dataset Information

In vivo T cell activation induces the formation of CD209(+) PDL-2(+) dendritic cells.

Publications

In vivo T cell activation induces the formation of CD209(+) PDL-2(+) dendritic cells.

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