Allele-specific detection of single mRNA molecules in situ.
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ABSTRACT:
SUBMITTER: Hansen CH
PROVIDER: S-EPMC3789122 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Allele-specific detection of single mRNA molecules in situ.
Hansen Clinton H CH van Oudenaarden Alexander A
Nature methods 20130811 9
We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells. ...[more]
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