Ontology highlight
ABSTRACT:
SUBMITTER: van Es JH
PROVIDER: S-EPMC3789123 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
van Es Johan H JH Sato Toshiro T van de Wetering Marc M Lyubimova Anna A Yee Nee Annie Ng AN Gregorieff Alex A Sasaki Nobuo N Zeinstra Laura L van den Born Maaike M Korving Jeroen J Martens Anton C M ACM Barker Nick N van Oudenaarden Alexander A Clevers Hans H
Nature cell biology 20120923 10
Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cel ...[more]