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Intracellular regulation of cross-presentation during dendritic cell maturation.

ABSTRACT: We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-?, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal/lysosomal acidification. Nevertheless, intra-phagosomal degradation of OVA was not increased in matured samples, suggesting that decreasing phagosomal pH may also regulate cross-presentation by a mechanism other than enhancing degradation.

SUBMITTER: Wagner CS

PROVIDER: S-EPMC3789698 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Intracellular regulation of cross-presentation during dendritic cell maturation.

Wagner Claudia S CS Grotzke Jeff J Cresswell Peter P

PloS one 20131003 10

We have investigated the effect of different maturation stimuli on the ability of mature dendritic cells (DCs) to cross-present newly acquired particulate antigens. Cross-presentation was impaired in DCs matured by treatment with TNF-α, CpG and LPS, but was less affected upon CD40L-induced maturation. The difference could not be explained by decreased antigen uptake or translocation into the cytosol, but decreased cross-presentation ability did correlate with increased phagosomal/lysosomal acidi ...[more]

PMID: 24098562

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