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RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells.

ABSTRACT: In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8?+ and CD103+ compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1- cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal development of cDCs but show a defect for in vivo and in vitro cross-presentation of cell-associated antigen. This defect is specific to cDCs, as Rab43-deficient monocyte-derived DCs showed no defect in cross-presentation of cell-associated antigen. These results suggest that RAB43 provides a specialized activity used in cross-presentation selectively by CD8?+ DCs but not other antigen-presenting cells.

SUBMITTER: Kretzer NM 

PROVIDER: S-EPMC5154939 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells.

Kretzer Nicole M NM   Theisen Derek J DJ   Tussiwand Roxane R   Briseño Carlos G CG   Grajales-Reyes Gary E GE   Wu Xiaodi X   Durai Vivek V   Albring Jörn J   Bagadia Prachi P   Murphy Theresa L TL   Murphy Kenneth M KM  

The Journal of experimental medicine 20161129 13

In this study, to examine cross-presentation by classical dendritic cells (DCs; cDCs), we evaluated the role of RAB43, a protein found to be selectively expressed by Batf3-dependent CD8α<sup>+</sup> and CD103<sup>+</sup> compared with other DC subsets and immune lineages. Using a specific monoclonal antibody, we localized RAB43 expression to the Golgi apparatus and LAMP1<sup>-</sup> cytoplasmic vesicles. Mice with germline or conditional deletion of Rab43 are viable and fertile and have normal d  ...[more]

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