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Interleukin-4 induces senescence in human renal carcinoma cell lines through STAT6 and p38 MAPK.

ABSTRACT: Interleukin (IL)-4, originally identified as a lymphocyte growth factor, can directly inhibit growth of certain tumor cell types. We reported previously that IL-4 induced cell cycle arrest in G1 phase through an increase in p21(WAF1/CIP1) expression in human renal cell carcinoma (RCC) cell lines. In the present study, we investigated the underlying mechanism of IL-4-induced growth inhibition. In four of six human RCC cell lines, including Caki-1, A498, SNU482, and SNU228, IL-4 induced cellular senescence as demonstrated by enlarged and flattened morphology, increased granularity, and senescence-associated-?-galactosidase (SA-?-gal) staining. Signal tranducer and activator of transcription 6 (STAT6) and p38 MAPK were found to mediate IL-4-induced growth inhibition and cellular senescence. Both of these molecules were activated by 10 min after IL-4 treatment, and inhibition of their activity or expression prevented growth suppression and cellular senescence induced by IL-4. Inhibiting or silencing either STAT6 or p38 MAPK alone partially reduced the effect of IL-4, whereas inhibiting or silencing both molecules exerted an additive effect and almost completely abrogated the effect of IL-4. Thus STAT6 and p38 MAPK appeared to independently mediate IL-4-induced growth inhibition and cellular senescence. The p21(WAF1/CIP1) up-regulation that accompanied growth inhibition and cellular senescence by IL-4 was also attenuated additively when p38 MAPK and STAT6 were silenced. Taken together, these results show that IL-4 induces cellular senescence through independent signaling pathways involving STAT6 and p38 MAPK in some human RCC cell lines.

SUBMITTER: Kim HD

PROVIDER: S-EPMC3789971 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Interleukin-4 induces senescence in human renal carcinoma cell lines through STAT6 and p38 MAPK.

Kim Hag Dong HD Yu Su-Jin SJ Kim Hee Suk HS Kim Yong-Jin YJ Choe Jeong Min JM Park Yun Gyu YG Kim Joon J Sohn Jeongwon J

The Journal of biological chemistry 20130809 40

Interleukin (IL)-4, originally identified as a lymphocyte growth factor, can directly inhibit growth of certain tumor cell types. We reported previously that IL-4 induced cell cycle arrest in G1 phase through an increase in p21(WAF1/CIP1) expression in human renal cell carcinoma (RCC) cell lines. In the present study, we investigated the underlying mechanism of IL-4-induced growth inhibition. In four of six human RCC cell lines, including Caki-1, A498, SNU482, and SNU228, IL-4 induced cellular s ...[more]

PMID: 23935100

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Dataset Information

Interleukin-4 induces senescence in human renal carcinoma cell lines through STAT6 and p38 MAPK.

Publications

Interleukin-4 induces senescence in human renal carcinoma cell lines through STAT6 and p38 MAPK.

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