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CAR regulates epithelial cell junction stability through control of E-cadherin trafficking.

ABSTRACT: CAR (Coxsackie and Adenovirus Receptor) is the primary docking receptor for typeB coxsackie viruses and subgroup C adenoviruses. CAR is a member of the JAM family of adhesion receptors and is located to both tight and adherens junctions between epithelial cells where it can assemble adhesive contacts through homodimerisation in trans. However, the role of CAR in controlling epithelial junction dynamics remains poorly understood. Here we demonstrate that levels of CAR in human epithelial cells play a key role in determining epithelial cell adhesion through control of E-cadherin stability at cell-cell junctions. Mechanistically, we show that CAR is phosphorylated within the C-terminus by PKC? and that this in turn controls Src-dependent endocytosis of E-cadherin at cell junctions. This data demonstrates a novel role for CAR in regulating epithelial homeostasis.

SUBMITTER: Morton PE

PROVIDER: S-EPMC3791454 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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CAR regulates epithelial cell junction stability through control of E-cadherin trafficking.

Morton Penny E PE Hicks Alexander A Nastos Theodoros T Santis George G Parsons Maddy M

Scientific reports 20131007

CAR (Coxsackie and Adenovirus Receptor) is the primary docking receptor for typeB coxsackie viruses and subgroup C adenoviruses. CAR is a member of the JAM family of adhesion receptors and is located to both tight and adherens junctions between epithelial cells where it can assemble adhesive contacts through homodimerisation in trans. However, the role of CAR in controlling epithelial junction dynamics remains poorly understood. Here we demonstrate that levels of CAR in human epithelial cells pl ...[more]

PMID: 24096322

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