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Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK.


ABSTRACT: Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a strong predictor for the development of hypertension, diabetes mellitus, and cardiovascular disease. This study examined the antiobesity effect of an ethanol extract of Corni Fructus containing formulation (CDAP), which is a combination of four natural components: Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix. The cellular lipid content in 3T3-L1 adipocytes was assessed by Oil Red O staining. Expressions of peroxisome proliferator-activated receptor- ? (PPAR- ? ), CCAAT/enhancer-binding protein- ? (C/EBP- ? ), and lipin-1 were determined by real-time RT-PCR. Western blot was used to determine the protein levels of PPAR- ? , C/EBP- ? , and AMP-activated protein kinase- ? (AMPK- ? ). The CDAP extract suppressed the differentiation of 3T3-L1 adipocytes by downregulating cellular induction of PPAR- ? , C/EBP- ? , and lipin-1. The CDAP extract also significantly upregulated phosphorylation of AMPK- ? . An in vivo study showed that CDAP induced weight loss in mice with high-fat-diet-induced obesity. These results indicate that CDAP has a potent anti-obesity effect due to the inhibition of adipocyte differentiation and adipogenesis.

SUBMITTER: Kim HL 

PROVIDER: S-EPMC3792538 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK.

Kim Hye-Lin HL   Jeon Yong-Deok YD   Park Jinbong J   Rim Hong-Kun HK   Jeong Mi-Young MY   Lim Hara H   Ko Seong-Gyu SG   Jang Hyeung-Jin HJ   Lee Byung-Cheol BC   Lee Kyung-Tae KT   Lee Kang-Min KM   Lee Hyejung H   Kim Sung-Hoon SH   Kim Su-Jin SJ   Hong Seung-Heon SH   Um Jae-Young JY  

Evidence-based complementary and alternative medicine : eCAM 20130919


Obesity is a metabolic disorder characterized by chronic inflammation and dyslipidemia and is a strong predictor for the development of hypertension, diabetes mellitus, and cardiovascular disease. This study examined the antiobesity effect of an ethanol extract of Corni Fructus containing formulation (CDAP), which is a combination of four natural components: Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix. The cellular lipid content in 3T3-L1 adipocytes was  ...[more]

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