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Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.

ABSTRACT: The metabolism of arachidonic acid (ARA) by cytochrome P450 ?/?-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the ?-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

SUBMITTER: Van Bogaert I

PROVIDER: S-EPMC3793626 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

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Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.

Van Bogaert Inge I Zhang Guodong G Yang Jun J Liu Jun-Yan JY Ye Yonghao Y Soetaert Wim W Hammock Bruce D BD

Journal of lipid research 20130821 11

The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to ...[more]

PMID: 23966667

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