Ontology highlight
ABSTRACT:
SUBMITTER: Li JX
PROVIDER: S-EPMC3794799 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Li Jun-Xia JX Zhao Long-Mei LM Wu Ru-Juan RJ Zheng Zhao-Jun ZJ Zhang Ri-Jun RJ
International journal of molecular sciences 20130910 9
In this study, we constructed a novel Streptomyces-E.coli shuttle vector pZRJ362 combining the xylose isomerase promoter and amylase terminator. A gene encoding the endoglucanase Cel6A in Thermobifida fusca was amplified by PCR, cloned into Streptomyces lividans host strain using the novel expression vector and Pichia pastoris GS115 host strain using the vector pPICZα-C, respectively. Afterwards, the expression pattern and the maximum expression level were comparatively studied in both expressio ...[more]