Ontology highlight
ABSTRACT:
SUBMITTER: Cong L
PROVIDER: S-EPMC3795411 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Cong Le L Ran F Ann FA Cox David D Lin Shuailiang S Barretto Robert R Habib Naomi N Hsu Patrick D PD Wu Xuebing X Jiang Wenyan W Marraffini Luciano A LA Zhang Feng F
Science (New York, N.Y.) 20130103 6121
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 ...[more]