Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.
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ABSTRACT:
SUBMITTER: Chiu H
PROVIDER: S-EPMC3813845 | biostudies-other | 2013 Nov
REPOSITORIES: biostudies-other
Publications
Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.
Chiu Hui H Schwartz Hillel T HT Antoshechkin Igor I Sternberg Paul W PW
Genetics 20130826 3
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific. ...[more]
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