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Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.


ABSTRACT: CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.

SUBMITTER: Chiu H 

PROVIDER: S-EPMC3813845 | biostudies-other | 2013 Nov

REPOSITORIES: biostudies-other

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Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.

Chiu Hui H   Schwartz Hillel T HT   Antoshechkin Igor I   Sternberg Paul W PW  

Genetics 20130826 3


CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific. ...[more]

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