Ontology highlight
ABSTRACT:
SUBMITTER: Wellensiek BP
PROVIDER: S-EPMC3795497 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Wellensiek Brian P BP Larsen Andrew C AC Flores Julia J Jacobs Bertram L BL Chaput John C JC
Protein science : a publication of the Protein Society 20130904 10
Many applications in biotechnology require human proteins generated from human cells. Stable cell lines commonly used for this purpose are difficult to develop, and scaling to large numbers of proteins can be problematic. Transient expression can circumvent this problem, but protein yields are generally too low for most applications. Here we report a novel 37-nucleotide leader sequence that promotes rapid and high transgene expression in mammalian cells. This sequence was identified by in vitro ...[more]