AMP sensing by DEAD-box RNA helicases.
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ABSTRACT:
SUBMITTER: Putnam AA
PROVIDER: S-EPMC3795843 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
AMP sensing by DEAD-box RNA helicases.
Putnam Andrea A AA Jankowsky Eckhard E
Journal of molecular biology 20130520 20
In eukaryotes, cellular levels of adenosine monophosphate (AMP) signal the metabolic state of the cell. AMP concentrations increase significantly upon metabolic stress, such as glucose deprivation in yeast. Here, we show that several DEAD-box RNA helicases are sensitive to AMP, which is not produced during ATP hydrolysis by these enzymes. We find that AMP potently inhibits RNA binding and unwinding by the yeast DEAD-box helicases Ded1p, Mss116p, and eIF4A. However, the yeast DEAD-box helicases S ...[more]
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