Ontology highlight
ABSTRACT:
SUBMITTER: Gao Z
PROVIDER: S-EPMC4990422 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Gao Zhaofeng Z Putnam Andrea A AA Bowers Heath A HA Guenther Ulf-Peter UP Ye Xuan X Kindsfather Audrey A Hilliker Angela K AK Jankowsky Eckhard E
eLife 20160805
Eukaryotic translation initiation involves two conserved DEAD-box RNA helicases, eIF4A and Ded1p. Here we show that S. cerevisiae eIF4A and Ded1p directly interact with each other and simultaneously with the scaffolding protein eIF4G. We delineate a comprehensive thermodynamic framework for the interactions between Ded1p, eIF4A, eIF4G, RNA and ATP, which indicates that eIF4A, with and without eIF4G, acts as a modulator for activity and substrate preferences of Ded1p, which is the RNA remodeling ...[more]