Ontology highlight
ABSTRACT:
SUBMITTER: Sander JD
PROVIDER: S-EPMC3799455 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Sander Jeffry D JD Ramirez Cherie L CL Linder Samantha J SJ Pattanayak Vikram V Shoresh Noam N Ku Manching M Foden Jennifer A JA Reyon Deepak D Bernstein Bradley E BE Liu David R DR Joung J Keith JK
Nucleic acids research 20130814 19
Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can confound interpretation of experiments and can have implications for development of therapeutic applications. Recently, two improved methods for identifying the off-target effects of zinc finger nuclease ...[more]