Ontology highlight
ABSTRACT:
SUBMITTER: Irague R
PROVIDER: S-EPMC3799614 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Irague Romain R Tarquis Laurence L André Isabelle I Moulis Claire C Morel Sandrine S Monsan Pierre P Potocki-Véronèse Gabrielle G Remaud-Siméon Magali M
PloS one 20131018 10
We used combinatorial engineering to investigate the relationships between structure and linkage specificity of the dextransucrase DSR-S from Leuconostoc mesenteroides NRRL B-512F, and to generate variants with altered specificity. Sequence and structural analysis of glycoside-hydrolase family 70 enzymes led to eight amino acids (D306, F353, N404, W440, D460, H463, T464 and S512) being targeted, randomized by saturation mutagenesis and simultaneously recombined. Screening of two libraries totali ...[more]