Unknown

Dataset Information

0

Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros.


ABSTRACT: C2H2 zinc fingers are found in several key transcriptional regulators in the immune system. However, these proteins usually contain more fingers than are needed for sequence-specific DNA binding, which suggests that different fingers regulate different genes and functions. Here we found that mice lacking finger 1 or finger 4 of Ikaros exhibited distinct subsets of the hematological defects of Ikaros-null mice. Most notably, the two fingers controlled different stages of lymphopoiesis, and finger 4 was selectively required for tumor suppression. The distinct defects support the hypothesis that only a small number of genes that are targets of Ikaros are critical for each of its biological functions. The subcategorization of functions and target genes by mutagenesis of individual zinc fingers will facilitate efforts to understand how zinc-finger transcription factors regulate development, immunity and disease.

SUBMITTER: Schjerven H 

PROVIDER: S-EPMC3800053 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Selective regulation of lymphopoiesis and leukemogenesis by individual zinc fingers of Ikaros.

Schjerven Hilde H   McLaughlin Jami J   Arenzana Teresita L TL   Frietze Seth S   Cheng Donghui D   Wadsworth Sarah E SE   Lawson Gregory W GW   Bensinger Steven J SJ   Farnham Peggy J PJ   Witte Owen N ON   Smale Stephen T ST  

Nature immunology 20130908 10


C2H2 zinc fingers are found in several key transcriptional regulators in the immune system. However, these proteins usually contain more fingers than are needed for sequence-specific DNA binding, which suggests that different fingers regulate different genes and functions. Here we found that mice lacking finger 1 or finger 4 of Ikaros exhibited distinct subsets of the hematological defects of Ikaros-null mice. Most notably, the two fingers controlled different stages of lymphopoiesis, and finger  ...[more]

Similar Datasets

2013-09-08 | GSE33693 | GEO
2013-09-08 | E-GEOD-33693 | biostudies-arrayexpress
2013-09-08 | E-GEOD-33679 | biostudies-arrayexpress
2013-09-08 | E-GEOD-32190 | biostudies-arrayexpress
2013-09-08 | GSE33679 | GEO
2013-09-08 | GSE32190 | GEO
2013-09-08 | E-GEOD-47496 | biostudies-arrayexpress
2013-09-08 | GSE47496 | GEO
2013-09-08 | E-GEOD-38841 | biostudies-arrayexpress
2013-09-08 | GSE38841 | GEO